Cryopreservation enhances embryogenic capacity of Gentiana cruciata (L.) suspension culture and maintains (epi)genetic uniformity of regenerants
Identifieur interne : 001A88 ( Main/Exploration ); précédent : 001A87; suivant : 001A89Cryopreservation enhances embryogenic capacity of Gentiana cruciata (L.) suspension culture and maintains (epi)genetic uniformity of regenerants
Auteurs : Anna Mikuła [Pologne] ; Karolina Tomiczak [Pologne] ; Jan J. Rybczy Ski [Pologne]Source :
- Plant Cell Reports [ 0721-7714 ] ; 2010.
Abstract
The embryogenic cell suspension culture of
Url:
DOI: 10.1007/s00299-010-0970-1
PubMed: 21161232
PubMed Central: 3056987
Affiliations:
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<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Cryopreservation enhances embryogenic capacity of <italic>Gentiana cruciata</italic> (L.) suspension culture and maintains (epi)genetic uniformity of regenerants</title><author><name sortKey="Mikula, Anna" sort="Mikula, Anna" uniqKey="Mikula A" first="Anna" last="Mikuła">Anna Mikuła</name><affiliation wicri:level="1"><nlm:aff id="Aff1">Botanical Garden–Center for Biological Diversity Conservation, Polish Academy of Sciences, ul. Prawdziwka 2, 02-973 Warsaw, Poland</nlm:aff><country xml:lang="fr">Pologne</country><wicri:regionArea>Botanical Garden–Center for Biological Diversity Conservation, Polish Academy of Sciences, ul. Prawdziwka 2, 02-973 Warsaw</wicri:regionArea><wicri:noRegion>02-973 Warsaw</wicri:noRegion></affiliation></author><author><name sortKey="Tomiczak, Karolina" sort="Tomiczak, Karolina" uniqKey="Tomiczak K" first="Karolina" last="Tomiczak">Karolina Tomiczak</name><affiliation wicri:level="1"><nlm:aff id="Aff1">Botanical Garden–Center for Biological Diversity Conservation, Polish Academy of Sciences, ul. Prawdziwka 2, 02-973 Warsaw, Poland</nlm:aff><country xml:lang="fr">Pologne</country><wicri:regionArea>Botanical Garden–Center for Biological Diversity Conservation, Polish Academy of Sciences, ul. Prawdziwka 2, 02-973 Warsaw</wicri:regionArea><wicri:noRegion>02-973 Warsaw</wicri:noRegion></affiliation></author><author><name sortKey="Rybczy Ski, Jan J" sort="Rybczy Ski, Jan J" uniqKey="Rybczy Ski J" first="Jan J." last="Rybczy Ski">Jan J. Rybczy Ski</name><affiliation wicri:level="1"><nlm:aff id="Aff1">Botanical Garden–Center for Biological Diversity Conservation, Polish Academy of Sciences, ul. Prawdziwka 2, 02-973 Warsaw, Poland</nlm:aff><country xml:lang="fr">Pologne</country><wicri:regionArea>Botanical Garden–Center for Biological Diversity Conservation, Polish Academy of Sciences, ul. Prawdziwka 2, 02-973 Warsaw</wicri:regionArea><wicri:noRegion>02-973 Warsaw</wicri:noRegion></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PMC</idno><idno type="pmid">21161232</idno><idno type="pmc">3056987</idno><idno type="url">http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3056987</idno><idno type="RBID">PMC:3056987</idno><idno type="doi">10.1007/s00299-010-0970-1</idno><date when="2010">2010</date><idno type="wicri:Area/Pmc/Corpus">000690</idno><idno type="wicri:Area/Pmc/Curation">000689</idno><idno type="wicri:Area/Pmc/Checkpoint">000F68</idno><idno type="wicri:Area/Ncbi/Merge">000C76</idno><idno type="wicri:Area/Ncbi/Curation">000C76</idno><idno type="wicri:Area/Ncbi/Checkpoint">000C76</idno><idno type="wicri:doubleKey">0721-7714:2010:Mikula A:cryopreservation:enhances:embryogenic</idno><idno type="wicri:Area/Main/Merge">001B25</idno><idno type="wicri:Area/Main/Curation">001A88</idno><idno type="wicri:Area/Main/Exploration">001A88</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en" level="a" type="main">Cryopreservation enhances embryogenic capacity of <italic>Gentiana cruciata</italic> (L.) suspension culture and maintains (epi)genetic uniformity of regenerants</title><author><name sortKey="Mikula, Anna" sort="Mikula, Anna" uniqKey="Mikula A" first="Anna" last="Mikuła">Anna Mikuła</name><affiliation wicri:level="1"><nlm:aff id="Aff1">Botanical Garden–Center for Biological Diversity Conservation, Polish Academy of Sciences, ul. Prawdziwka 2, 02-973 Warsaw, Poland</nlm:aff><country xml:lang="fr">Pologne</country><wicri:regionArea>Botanical Garden–Center for Biological Diversity Conservation, Polish Academy of Sciences, ul. Prawdziwka 2, 02-973 Warsaw</wicri:regionArea><wicri:noRegion>02-973 Warsaw</wicri:noRegion></affiliation></author><author><name sortKey="Tomiczak, Karolina" sort="Tomiczak, Karolina" uniqKey="Tomiczak K" first="Karolina" last="Tomiczak">Karolina Tomiczak</name><affiliation wicri:level="1"><nlm:aff id="Aff1">Botanical Garden–Center for Biological Diversity Conservation, Polish Academy of Sciences, ul. Prawdziwka 2, 02-973 Warsaw, Poland</nlm:aff><country xml:lang="fr">Pologne</country><wicri:regionArea>Botanical Garden–Center for Biological Diversity Conservation, Polish Academy of Sciences, ul. Prawdziwka 2, 02-973 Warsaw</wicri:regionArea><wicri:noRegion>02-973 Warsaw</wicri:noRegion></affiliation></author><author><name sortKey="Rybczy Ski, Jan J" sort="Rybczy Ski, Jan J" uniqKey="Rybczy Ski J" first="Jan J." last="Rybczy Ski">Jan J. Rybczy Ski</name><affiliation wicri:level="1"><nlm:aff id="Aff1">Botanical Garden–Center for Biological Diversity Conservation, Polish Academy of Sciences, ul. Prawdziwka 2, 02-973 Warsaw, Poland</nlm:aff><country xml:lang="fr">Pologne</country><wicri:regionArea>Botanical Garden–Center for Biological Diversity Conservation, Polish Academy of Sciences, ul. Prawdziwka 2, 02-973 Warsaw</wicri:regionArea><wicri:noRegion>02-973 Warsaw</wicri:noRegion></affiliation></author></analytic><series><title level="j">Plant Cell Reports</title><idno type="ISSN">0721-7714</idno><idno type="eISSN">1432-203X</idno><imprint><date when="2010">2010</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><p>The embryogenic cell suspension culture of <italic>Gentiana cruciata,</italic> cryopreserved by the encapsulation/dehydration method, survived both short- (48 h) and long-term (1.5 years) cryostorage with more than 80% viability. To assess the influence of cryotreatments on the embryogenic potential, a proembryogenic mass was encapsulated and exposed to the following treatments: (1) osmotic dehydration (OD), (2) OD + air desiccation (AD) and (3) OD + AD + cryostorage (LN). The somatic embryogenesis efficiency increased ten times after osmotic dehydration. The AD and LN cryotreatments did not cause any significant alterations in somatic embryo production. We monitored the (epi)genetic stability of 288 regenerants derived from: non-cryotreated, short-term, and long-term cryostored tissue using metAFLP markers and ten primer combinations. Changes in the sequence and DNA methylation levels were studied by subjecting the DNA to digestion with two pairs of isoschisomer restriction enzymes (<italic>Kpn</italic>I/<italic>Mse</italic>I and <italic>Acc65</italic>I/<italic>Ms</italic>eI). Two new AFLP unique DNA fragments at the DNA sequence level, with no differences at the methylation level, were found between regenerants derived from cryopreserved tissue, compared with the non-cryotreated controls. The <italic>Acc65</italic>I/<italic>Ms</italic>eI methylation levels for the three groups of regenerants were not significantly different. Cluster analysis was capable of identifying a number of sub-clusters. Only one of the sub-clusters comprises almost all regenerants derived from non-cryotreated and short-term cryostored tissue. Plantlets derived from long-term cryostored tissue were grouped into separate clusters. The observed AFLP alterations did not appear to be associated with the use of cryopreservation, but were probably related to the process of in vitro culture.</p></div></front><back><div1 type="bibliography"><listBibl><biblStruct><analytic><author><name sortKey="Aguilar, Me" uniqKey="Aguilar M">ME Aguilar</name></author><author><name sortKey="Engelmann, F" uniqKey="Engelmann F">F Engelmann</name></author><author><name sortKey="Michaux Ferriere, N" uniqKey="Michaux Ferriere N">N Michaux-Ferrière</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Ahuja, S" uniqKey="Ahuja S">S Ahuja</name></author><author><name sortKey="Mandal, Bb" uniqKey="Mandal B">BB Mandal</name></author><author><name sortKey="Dixit, S" uniqKey="Dixit S">S Dixit</name></author><author><name sortKey="Srivastava, Ps" uniqKey="Srivastava P">PS Srivastava</name></author></analytic></biblStruct><biblStruct></biblStruct><biblStruct><analytic><author><name sortKey="Channuntapipat, C" 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Tanne</name></author><author><name sortKey="Perl, A" uniqKey="Perl A">A Perl</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Winkelmann, T" uniqKey="Winkelmann T">T Winkelmann</name></author><author><name sortKey="Mu Mann, V" uniqKey="Mu Mann V">V Mußmann</name></author><author><name sortKey="Serek, M" uniqKey="Serek M">M Serek</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Zaghmout, Omf" uniqKey="Zaghmout O">OMF Zaghmout</name></author><author><name sortKey="Torello, Wa" uniqKey="Torello W">WA Torello</name></author></analytic></biblStruct></listBibl></div1></back></TEI><affiliations><list><country><li>Pologne</li></country></list><tree><country name="Pologne"><noRegion><name sortKey="Mikula, Anna" sort="Mikula, Anna" uniqKey="Mikula A" first="Anna" last="Mikuła">Anna Mikuła</name></noRegion><name sortKey="Rybczy Ski, Jan J" sort="Rybczy Ski, Jan J" uniqKey="Rybczy Ski J" first="Jan J." last="Rybczy Ski">Jan J. Rybczy Ski</name><name sortKey="Tomiczak, Karolina" sort="Tomiczak, Karolina" uniqKey="Tomiczak K" first="Karolina" last="Tomiczak">Karolina Tomiczak</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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HfdIndexSelect -h $EXPLOR_AREA/Data/Main/Exploration/RBID.i -Sk "pubmed:21161232" \
| HfdSelect -Kh $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd \
| NlmPubMed2Wicri -a OrangerV1
| This area was generated with Dilib version V0.6.25. |  |